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G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was
As estimated using Ellman procedure [30] as modified by Gorun et al. [31] for the assay of purified preparations of cholinesterases. This procedure isThe LC 50 (30 minutes) for leaded (G1) was found to be 37475 ppm and for unleaded (G2) 39928 ppm. The rats of each group were subjected to aggressive behaviour tests for ten days, through the last ten days of exposure. After the completion of this t
D the colour was developed upon the addition of DTNB - Phosphate - ethanol reagent. The colour was then read immediately at 412 nm and the enzyme activity was determined in M per SH group (MSH) from a standard curve.Determination of total proteins The total protein content was determined according to the method described by Lowry et al. [32]. This method is based on the reaction between the trypt
Idly adducting his extended fore limbs and he also bites the ear or limb or the tail. Boxing position: longer intervals in which both rats adopt a defensive attitude.B- Physiological and biochemical measurements Determination of superoxide dismutase (SOD) activity Superoxide dismutase (SOD) activity was assayed by the procedure of Niskikimi et al. [26]. The assay relies on the ability of the enz
Up as compared with the corresponding values of the control. Data presented in tables 3 show that the rats exposed to the two types of vapours encountered a significant decrease in the norepinephrine, dopamine and serotonin contents of the hippocampus as compared with the control group. On other hand, no significant changes were found in norepinephrine and serotonin between the leaded or the unle
Ne groups. On the other hand, no statistical difference was found between the control and the leaded groups. Serotonin content was not affected in all the three experimental groups when comparison was made between each other. In the hypothalamus, data in table 2 reveal that the norepinephrine, dopamine and serotonin in the group exposed to the unleaded gasoline were lower than those of the contro
Nd norepinephrine were elevated above the control level. Data in table 5 reveal that Na+, K+-ATPase activity and total protein content in the two groups exposed to gasoline were lower than the control, although no significant difference was found between each other. The rats exposed to the unleaded gasoline had a significantly lower acetylcholinesterase activity as compared with the control or le
Up as compared with the corresponding values of the control. Data presented in tables 3 show that the rats exposed to the two types of vapours encountered a significant decrease in the norepinephrine, dopamine and serotonin contents of the hippocampus as compared with the control group. On other hand, no significant changes were found in norepinephrine and serotonin between the leaded or the unle
Up as compared with the corresponding values of the control. Data presented in tables 3 show that the rats exposed to the two types of vapours encountered a significant decrease in the norepinephrine, dopamine and serotonin contents of the hippocampus as compared with the control group. On other hand, no significant changes were found in norepinephrine and serotonin between the leaded or the unle
Le of the assay depends on the incubation of adenosine triphosphate with the tissue-containing enzyme and the determination of liberated inorganic phosphorus as modified by El-Aaser and El-Merzabani [33]. Statistical analysis The data were subjected to statistical analysis using one way ANOVA. SPSS for Windows software, Release 11.0 (SPSS, Chicago, IL) was used.ResultsData in table 1 show that th
Oline Norepinephrine 0.115 ?0.031 0.11 ?0.013 0.076 ?0.0038 a** b** Dopamine 2.68 ?0.27 1.136 ?0.29 a*** 1.61 ?0.28 a*** b* Serotonin 0.822 ?0.047 0.50 ?0.075 a*** 0.49 ?0.13 a***Values are expressed as means ?SD a: significantly different from the control group. b: significantly different from the leaded gasoline. Asterisks indicate the level of significance (* P
G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was
G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was
G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was


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